basolateral culture media Search Results


c-myc antibody  (NSJ Bioreagents)
99
NSJ Bioreagents c-myc antibody
C Myc Antibody, supplied by NSJ Bioreagents, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/c-myc antibody/product/NSJ Bioreagents
Average 99 stars, based on 1 article reviews
c-myc antibody - by Bioz Stars, 2026-03
99/100 stars
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il 6  (Thermo Fisher)
86
Thermo Fisher il 6
Neutrophil extracellular trap (NET)-mediated injury to airway epithelial cells (AECs). (A) Apical exposure of AECs grown at air–liquid interface (ALI) to NETs (6 μg/ml) for 24 hours causes upregulation of gene expression <t>for</t> <t>IL-6</t> and IL-8 (data are from epithelial cells from seven donors). (B) Apical exposure of AECs grown at ALI to NETs (6 μg/ml) for 24 hours causes release of IL-6 and IL-8 protein into the conditioned media (data are from epithelial cells from seven donors). (C) Glucose-6-phosphate dehydrogenase (G6PD) release into conditioned media of AECs in ALI culture after exposure to NETs (6 μg/ml) at the apical surface for 24 hours in the presence or absence of DNase I cotreatment (data are from epithelial cells from five donors). Error bars represent SEM. *P < 0.05 and ***P < 0.0001. Circles represent individual data points. RFU = relative fluorescence units.
Il 6, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/il 6/product/Thermo Fisher
Average 86 stars, based on 1 article reviews
il 6 - by Bioz Stars, 2026-03
86/100 stars
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basolateral culture media  (MatTek)
90
MatTek basolateral culture media
Neutrophil extracellular trap (NET)-mediated injury to airway epithelial cells (AECs). (A) Apical exposure of AECs grown at air–liquid interface (ALI) to NETs (6 μg/ml) for 24 hours causes upregulation of gene expression <t>for</t> <t>IL-6</t> and IL-8 (data are from epithelial cells from seven donors). (B) Apical exposure of AECs grown at ALI to NETs (6 μg/ml) for 24 hours causes release of IL-6 and IL-8 protein into the conditioned media (data are from epithelial cells from seven donors). (C) Glucose-6-phosphate dehydrogenase (G6PD) release into conditioned media of AECs in ALI culture after exposure to NETs (6 μg/ml) at the apical surface for 24 hours in the presence or absence of DNase I cotreatment (data are from epithelial cells from five donors). Error bars represent SEM. *P < 0.05 and ***P < 0.0001. Circles represent individual data points. RFU = relative fluorescence units.
Basolateral Culture Media, supplied by MatTek, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/basolateral culture media/product/MatTek
Average 90 stars, based on 1 article reviews
basolateral culture media - by Bioz Stars, 2026-03
90/100 stars
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triton x-100  (Millipore)
90
Millipore triton x-100
Neutrophil extracellular trap (NET)-mediated injury to airway epithelial cells (AECs). (A) Apical exposure of AECs grown at air–liquid interface (ALI) to NETs (6 μg/ml) for 24 hours causes upregulation of gene expression <t>for</t> <t>IL-6</t> and IL-8 (data are from epithelial cells from seven donors). (B) Apical exposure of AECs grown at ALI to NETs (6 μg/ml) for 24 hours causes release of IL-6 and IL-8 protein into the conditioned media (data are from epithelial cells from seven donors). (C) Glucose-6-phosphate dehydrogenase (G6PD) release into conditioned media of AECs in ALI culture after exposure to NETs (6 μg/ml) at the apical surface for 24 hours in the presence or absence of DNase I cotreatment (data are from epithelial cells from five donors). Error bars represent SEM. *P < 0.05 and ***P < 0.0001. Circles represent individual data points. RFU = relative fluorescence units.
Triton X 100, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/triton x-100/product/Millipore
Average 90 stars, based on 1 article reviews
triton x-100 - by Bioz Stars, 2026-03
90/100 stars
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Image Search Results


Neutrophil extracellular trap (NET)-mediated injury to airway epithelial cells (AECs). (A) Apical exposure of AECs grown at air–liquid interface (ALI) to NETs (6 μg/ml) for 24 hours causes upregulation of gene expression for IL-6 and IL-8 (data are from epithelial cells from seven donors). (B) Apical exposure of AECs grown at ALI to NETs (6 μg/ml) for 24 hours causes release of IL-6 and IL-8 protein into the conditioned media (data are from epithelial cells from seven donors). (C) Glucose-6-phosphate dehydrogenase (G6PD) release into conditioned media of AECs in ALI culture after exposure to NETs (6 μg/ml) at the apical surface for 24 hours in the presence or absence of DNase I cotreatment (data are from epithelial cells from five donors). Error bars represent SEM. *P < 0.05 and ***P < 0.0001. Circles represent individual data points. RFU = relative fluorescence units.

Journal: American Journal of Respiratory and Critical Care Medicine

Article Title: Extracellular DNA, Neutrophil Extracellular Traps, and Inflammasome Activation in Severe Asthma

doi: 10.1164/rccm.201810-1869OC

Figure Lengend Snippet: Neutrophil extracellular trap (NET)-mediated injury to airway epithelial cells (AECs). (A) Apical exposure of AECs grown at air–liquid interface (ALI) to NETs (6 μg/ml) for 24 hours causes upregulation of gene expression for IL-6 and IL-8 (data are from epithelial cells from seven donors). (B) Apical exposure of AECs grown at ALI to NETs (6 μg/ml) for 24 hours causes release of IL-6 and IL-8 protein into the conditioned media (data are from epithelial cells from seven donors). (C) Glucose-6-phosphate dehydrogenase (G6PD) release into conditioned media of AECs in ALI culture after exposure to NETs (6 μg/ml) at the apical surface for 24 hours in the presence or absence of DNase I cotreatment (data are from epithelial cells from five donors). Error bars represent SEM. *P < 0.05 and ***P < 0.0001. Circles represent individual data points. RFU = relative fluorescence units.

Article Snippet: Measurement of IL-6 and IL-8 was performed in 50 μl of basolateral conditioned media or blank cell culture media using the IL-6 and IL-8 Human ProcartaPlex Simplex Kits, Platinum (EPXP010-10213-901 and EPXP010-10204-901; Thermo Fisher Scientific) according to the manufacturer’s directions.

Techniques: Expressing, Fluorescence